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xct slc7a11  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc xct slc7a11
    Xct Slc7a11, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+slc7a11/xCT%2FSLC7A11+Antibody/10__3390_slash_antiox15101300-96-32-34
    Average 96 stars, based on 164 article reviews
    xct slc7a11 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Ginkgolic acid targets HSPA8 to trigger ferroptosis in hepatocellular carcinoma via chaperone-mediated autophagy-dependent GPX4 degradation
    Article Snippet: The following antibodies were used: anti-GPX4 (Immunoway, PT0621R, 1:1000, AB_3716555), anti-HSPA8 (Huabio, ET1602-33, 1:1000, AB_3069646), anti-ACTB (Huabio, HA722023, 1:20000, AB_3096833), anti-ACSL4 (Abcam, ab205197, 1:1000, AB_3716556), anti-SLC7A11 (CST, 12691, 1:1000, AB_2687474), anti-ATG5 (Huabio, ET1611-38, 1:4000, AB_3070016), anti-ATG7 (Huabio, ET1610-53, 1:4000, AB_3069938), anti-LAMP2A (Huabio, ET1601-24, 1:1000, AB_3069607), anti-Myc-tag (CST, 2272, 1:1000, AB_10692100), anti-Flag-tag (Huabio, 0912-1,1:1000, AB_3068715), anti-rabbit IgG HRP (Huabio, HA1001,1:10000, AB_2819166).

    Article Title: p53 and fatty acids collaborate to trigger ferroptosis via the FBXO2-FABP5 axis in colorectal cancer.
    Article Snippet: The antip21 (#2947), anti-SLC7A11 (#98051), anti-PUMA (#4976), anti-HA (#3724) antibody and the normal rabbit IgG (#2729) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: p53 and fatty acids collaborate to trigger ferroptosis via the FBXO2-FABP5 axis in colorectal cancer
    Article Snippet: The anti-Flag antibody (F1804) was purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Article Title: APR-246 drives ROS-dependent ferroptosis and apoptosis and enhances anti-PD-1 efficacy in bladder cancer.
    Article Snippet: Primary antibodies were as follows: anti-SLC7A11 (cat. no. 98051S), anti-GPX4 (cat. no. 52455S), and anti-PARP (cat. no. 9532S) (Cell Signaling Technology, USA); anti-p53 (cat. no. 14681-1-AP), anti-p21 (cat. no. 10355-1-AP), and anti-GAPDH (cat. no. 81640-5-RR) (Proteintech, Wuhan, China).

    Article Title: TCF3 activates super-enhancer-driven TRIB2 overexpression to suppress ferroptosis and promote hepatoblastoma proliferation.
    Article Snippet: The primary antibodies used in this study were anti-TRIB2 (1:1000, #13533S, Cell Signaling Technology, Danvers, MA, USA), anti-TCF3 (1:1000, #67140-1- Ig, Proteintech), anti-NRF2 (1:1000, #T55136S, Abmart, Shanghai, China), anti-KEAP1 (1:500, #TA5266S, Abmart), anti-GAPDH (1:5000, #60004-1-Ig, Proteintech, Wuhan, China), anti-NQO1 (1:1000, #ab28947, Abcam, Cambridge, UK), anti-HO-1 (1:1000, #10701-1-AP, Proteintech), anti-4-Hydroxynonenal (4-HNE, 1:1000, #ab46545, Abcam), anti-BACH1 (1:1000, #ab180853, Abcam), anti-p62 (1:5000, #ab109012, Abcam), antiFTH (1:1000, #ab314244, Abcam), anti-DMT1 (1:1000, #20507-1-AP, Proteintech), anti-SLC7A11 (1:2000, #12691S, Cell Signaling Technology), anti-TFRC (1:5000, #ab269513, Abcam), anti-GPX4 (1:1000, #67763-1-Ig, Proteintech), anti-ACSL4 (1:5000, #22401-1-AP, Proteintech), anti-α-tubulin (1:5000, #66031-1-Ig, Proteintech), anti-Histone H3 (1:1000, #9715S, Cell Signaling Technology), anti-Ubiquitin (1:1000, #3936S, Cell Signaling Technology), anti-Ubiquitin (linkage-specific K63; 1:1000, #ab179434, Abcam), and anti-Ubiquitin (linkage-specific K48; 1:1000, #ab140601, Abcam).

    Western Blot:

    Article Title: Phase Separation of NFIB Suppresses SLC3A2-Mediated Ferroptosis in Castration-Resistant Prostate Cancer.
    Article Snippet: .. The primary antibodies used in Western blotting included anti-NFIB (1:1000, Abcam), anti-SLC3A2 (1:1000, CST), anti-SLC7A11 (1:1000, CST), anti-GAPDH (1:5000, proteintech), anti-Ace-lys (1:1000, CST). ..

    Plasmid Preparation:

    Article Title: HER2-dependent paraptosis and ferroptosis induction by cannabidiol in breast cancer cells.
    Article Snippet: HER2 (human epidermal growth factor receptor 2) is a well-established oncogenic driver and therapeutic target in breast cancer.. Cannabidiol (CBD), a non-psychoactive phytocannabinoid, has demonstrated anticancer potential, yet its mechanisms of action in HER2-positive breast cancer remain insufficiently characterized.. In this study, we examined the effects of CBD on HER2-positive (SK-BR-3, BT-474) and HER2-negative (MCF-7, MDAMB-231) breast cancer cell lines, with a focus on its interaction with HER2.

    Cell-Signaling:

    Article Title: Elevated SLC3A2 Expression Promotes the Progression of Gliomas and Enhances Ferroptosis Resistance through the AKT/NRF2/GPX4 Axis
    Article Snippet: The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Biosharp) supplemented with 10% fetal bovine serum (ExCell) and penicillin-streptomycin solution (Solarbio) at a 37°C CO 2 incubator. .. The antibodies used in this study included anti-CD98 (PA5-96401, Invitrogen), anti-SLC7A11 (12691S, Cell Signaling Technology), anti-GPX4 (ab125066, Abcam), anti-AKT1/2/3 (T55561F, Abmart), anti–phospho-AKT (Ser473) (T40067F, Abmart), EMT Marker/Epithelial to Mesenchymal Transition Marker (ab216833, Abcam), anti-Nrf2 (ab62352, Abcam), anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 6000-1-lg, Proteintech), anti–β-tubulin ( M20005 , Abmart). .. The AKT activator SC79 (SF2730) was purchased from Beyotime (Beijing).

    Marker:

    Article Title: Elevated SLC3A2 Expression Promotes the Progression of Gliomas and Enhances Ferroptosis Resistance through the AKT/NRF2/GPX4 Axis
    Article Snippet: The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Biosharp) supplemented with 10% fetal bovine serum (ExCell) and penicillin-streptomycin solution (Solarbio) at a 37°C CO 2 incubator. .. The antibodies used in this study included anti-CD98 (PA5-96401, Invitrogen), anti-SLC7A11 (12691S, Cell Signaling Technology), anti-GPX4 (ab125066, Abcam), anti-AKT1/2/3 (T55561F, Abmart), anti–phospho-AKT (Ser473) (T40067F, Abmart), EMT Marker/Epithelial to Mesenchymal Transition Marker (ab216833, Abcam), anti-Nrf2 (ab62352, Abcam), anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 6000-1-lg, Proteintech), anti–β-tubulin ( M20005 , Abmart). .. The AKT activator SC79 (SF2730) was purchased from Beyotime (Beijing).



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    Ferroptosis-specific validation of GINS1 knockdown-induced cell death in neuroblastoma cells. (A) Western blot analysis of GPX4 and SLC7A11 protein levels in SH-SY5Y and SK-N-SH cells transfected with shNC, shGINS1-1, and shGINS1-2. (B-C) CCK-8 assay assessing cell viability in SH-SY5Y (B) and SK-N-SH (C) cells transfected with shNC or shGINS1, treated with erastin (5 μM), RSL3 (1 μM), and/or ferrostatin-1 (Fer-1, 1 μM) as indicated. (D-E) Glutathione (GSH) levels in SH-SY5Y (D) and SK-N-SH (E) cells under the indicated conditions. (F-G) Intracellular ROS levels in SH-SY5Y (F) and SK-N-SH (G) cells under the indicated conditions. (H-I) Malondialdehyde (MDA) content in SH-SY5Y (H) and SK-N-SH (I) cells under the indicated conditions. (J-K) Fe 2+ levels in SH-SY5Y (J) and SK-N-SH (K) cells under the indicated conditions. Data are presented as mean ± SD from three independent experiments. Statistical significance was determined using one-way ANOVA. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.

    Journal: Molecular & Cellular Oncology

    Article Title: GINS1 suppresses ferroptosis by inhibiting the Hippo pathway in neuroblastoma

    doi: 10.1080/23723556.2026.2715868

    Figure Lengend Snippet: Ferroptosis-specific validation of GINS1 knockdown-induced cell death in neuroblastoma cells. (A) Western blot analysis of GPX4 and SLC7A11 protein levels in SH-SY5Y and SK-N-SH cells transfected with shNC, shGINS1-1, and shGINS1-2. (B-C) CCK-8 assay assessing cell viability in SH-SY5Y (B) and SK-N-SH (C) cells transfected with shNC or shGINS1, treated with erastin (5 μM), RSL3 (1 μM), and/or ferrostatin-1 (Fer-1, 1 μM) as indicated. (D-E) Glutathione (GSH) levels in SH-SY5Y (D) and SK-N-SH (E) cells under the indicated conditions. (F-G) Intracellular ROS levels in SH-SY5Y (F) and SK-N-SH (G) cells under the indicated conditions. (H-I) Malondialdehyde (MDA) content in SH-SY5Y (H) and SK-N-SH (I) cells under the indicated conditions. (J-K) Fe 2+ levels in SH-SY5Y (J) and SK-N-SH (K) cells under the indicated conditions. Data are presented as mean ± SD from three independent experiments. Statistical significance was determined using one-way ANOVA. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.

    Article Snippet: Membranes were incubated with primary antibodies against ACSL4 (1:2000, Proteintech, 22401-1-AP), p -YAP (1:1000, Proteintech, 80694-2-RR), YAP (1:1000, Proteintech, 13584-1-AP), AIFM2 (1:1000, Proteintech, 20886-1-AP), p -MST1 (1:1000, Cell Signaling Technology, #3681S), MST1 (1:1000, Cell Signaling Technology, #3682S), p -LATS1 (1:1000, Cell Signaling Technology, #8654S), LATS1 (1:1000, Cell Signaling Technology, #3477S), GPX4 (1:1000, Proteintech, 67763-1-Ig), SLC7A11 (1:1000, Proteintech, 26864-1-AP) and GAPDH (1:30000, Proteintech, 60004-1-lg), followed by HRP-conjugated secondary antibodies.

    Techniques: Biomarker Discovery, Knockdown, Western Blot, Analysis, Transfection, CCK-8 Assay